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Plasma membrane associated transcription of cytoplasmic DNA

Academic Article
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Overview

authors

  • Cheng, J. L.
  • Torkamani, Ali
  • Peng, Y. J.
  • Jones, T. M.
  • Lerner, Richard

publication date

  • July 2012

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Cytoplasmic membrane-associated DNA (cmDNA) is a species of DNA that attaches to the plasma membrane and has physical and chemical properties that differ from those of bulk chromosomal and mitochondrial DNAs. Here, we used deep sequencing to analyze cmDNA and showed that satellite DNAs consisting of both of simple (CCATT)(N) repeats from the pericentromere regions of the chromosomes and 171-bp α-satellite repeat sequences from centromeres were highly enriched. Importantly, we found there is a special cytoplasmic membrane-associated transcription system in which DNA-dependent RNA polymerase II, which colocalizes with template cmDNA at the plasma membrane, can transcribe the membrane-associated 171-bp α-satellite repeat sequences into RNA. Analysis of phosphorylation patterns indicated that the RNA polymerase II in the plasma membrane is in a different chemical state from its nuclear counterpart.

subject areas

  • B-Lymphocytes
  • Base Sequence
  • Cell Line
  • Cell Membrane
  • Centromere
  • Chromosomes
  • Cytoplasm
  • DNA, Satellite
  • Humans
  • Molecular Sequence Data
  • RNA Polymerase II
  • RNA, Messenger
  • RNA, Ribosomal
  • Transcription, Genetic
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Research

keywords

  • extranuclear coding
  • nongenomic gene expression
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Identity

PubMed Central ID

  • PMC3390847

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1208716109

PubMed ID

  • 22711823
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Additional Document Info

start page

  • 10827

end page

  • 10831

volume

  • 109

issue

  • 27

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