Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Interleukin 12 gene therapy of cancer by peritumoral injection of transduced autologous fibroblasts: Outcome of a phase I study

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Kang, W. K.
  • Park, C.
  • Yoon, H. L.
  • Kim, W. S.
  • Yoon, S. S.
  • Lee, M. H.
  • Park, K.
  • Kim, K.
  • Jeong, H. S.
  • Kim, J. A.
  • Nam, S. J.
  • Yang, J. H.
  • Son, Y. I.
  • Baek, C. H.
  • Han, Jiahuai
  • Ree, H. J.
  • Lee, E. S.
  • Kim, S. H.
  • Kim, Dae Hee
  • Ahn, Y. C.
  • Huh, S. J.
  • Choe, Y. H.
  • Lee, Jiing-Dwan
  • Park, M. H.
  • Kong, G. S.
  • Park, E. Y.
  • Kang, Y. K.
  • Bang, Y. J.
  • Paik, N. S.
  • Lee, S. N.
  • Kim, S.
  • Robbins, Paul D.
  • Tahara, H.
  • Lotze, M. T.
  • Park, C. H.

publication date

  • April 2001

journal

  • Human Gene Therapy  Journal

abstract

  • A phase I dose-escalation clinical trial of peritumoral injections of interleukin 12 (IL-12)-transduced autologous fibroblasts was performed in patients with disseminated cancer for whom effective treatment does not exist. The goals of this study were to assess the safety and toxicities as well as the efficacy, and ancillarily the immunomodulatory effects, of peritumoral IL-12 gene transfer. Primary dermal fibroblasts cultured from the patients were transduced with retroviral vector carrying human IL-12 genes (p35 and p40) as well as the neomycin phosphotransferase gene (TFG-hIL-12-Neo). Patients received four injections at intervals of 7 days. Nine patients were enrolled in this dose-escalation study, with secreted IL-12 doses ranging from 300 ng/24 hr for the first three patients to 1000, 3000, and 5000 ng/24 hr for two patients in each subsequent dosage level. Although a definite statement cannot be made, there appears to be perturbation of systemic immunity. Also, the locoregional effects mediated by tumor necrosis factor alpha (TNF-alpha) and CD8+ T cells were observed with tumor regression. Treatment-related adverse events were limited to mild to moderate pain at the injection site; clinically significant toxicities were not encountered. Transient but clear reductions of tumor sizes were observed at the injected sites in four of nine cases, and at noninjected distant sites in one melanoma patient. Hemorrhagic necrosis of tumors was observed in two melanoma patients. These data indicate that gene therapy by peritumoral injection of IL-12-producing autologous fibroblasts is feasible, and promising in patients with advanced cancer.

subject areas

  • Adult
  • CD8-Positive T-Lymphocytes
  • Female
  • Fibroblasts
  • Gene Transfer Techniques
  • Genetic Therapy
  • Humans
  • Immunoenzyme Techniques
  • Interleukin-12
  • Killer Cells, Natural
  • Male
  • Melanoma
  • Middle Aged
  • Retroviridae
  • Skin Neoplasms
  • T-Lymphocytes
  • Transforming Growth Factor beta
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1043-0342

Digital Object Identifier (DOI)

  • 10.1089/104303401300057388

PubMed ID

  • 11426466
scroll to property group menus

Additional Document Info

start page

  • 671

end page

  • 684

volume

  • 12

issue

  • 6

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support