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Biased signaling pathways in betaβ2-adrenergic receptor characterized by 19F-NMR

Academic Article
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Overview

related to degree

  • Liu, Jeffrey Jingyun, Ph.D. in Biology, Scripps Research 2007 - 2013

authors

  • Liu, Jeffrey Jingyun
  • Horst, R.
  • Katritch, Vsevolod
  • Stevens, Raymond
  • Wuthrich, Kurt

publication date

  • March 2012

journal

  • Science  Journal

abstract

  • Extracellular ligand binding to G protein-coupled receptors (GPCRs) modulates G protein and ?-arrestin signaling by changing the conformational states of the cytoplasmic region of the receptor. Using site-specific (19)F-NMR (fluorine-19 nuclear magnetic resonance) labels in the ?(2)-adrenergic receptor (?(2)AR) in complexes with various ligands, we observed that the cytoplasmic ends of helices VI and VII adopt two major conformational states. Changes in the NMR signals reveal that agonist binding primarily shifts the equilibrium toward the G protein-specific active state of helix VI. In contrast, ?-arrestin-biased ligands predominantly impact the conformational states of helix VII. The selective effects of different ligands on the conformational equilibria involving helices VI and VII provide insights into the long-range structural plasticity of ?(2)AR in partial and biased agonist signaling.
  • Extracellular ligand binding to G protein-coupled receptors (GPCRs) modulates G protein and β-arrestin signaling by changing the conformational states of the cytoplasmic region of the receptor. Using site-specific (19)F-NMR (fluorine-19 nuclear magnetic resonance) labels in the β(2)-adrenergic receptor (β(2)AR) in complexes with various ligands, we observed that the cytoplasmic ends of helices VI and VII adopt two major conformational states. Changes in the NMR signals reveal that agonist binding primarily shifts the equilibrium toward the G protein-specific active state of helix VI. In contrast, β-arrestin-biased ligands predominantly impact the conformational states of helix VII. The selective effects of different ligands on the conformational equilibria involving helices VI and VII provide insights into the long-range structural plasticity of β(2)AR in partial and biased agonist signaling.

subject areas

  • Adrenergic beta-2 Receptor Agonists
  • Arrestins
  • Binding Sites
  • Carbazoles
  • Cytoplasm
  • Drug Partial Agonism
  • Fluorine
  • Isoetharine
  • Isoproterenol
  • Ligands
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Propanolamines
  • Protein Conformation
  • Protein Structure, Secondary
  • Receptors, Adrenergic, beta-2
  • Signal Transduction
  • Structure-Activity Relationship
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Identity

PubMed Central ID

  • PMC3292700

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.1215802

PubMed ID

  • 22267580
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Additional Document Info

start page

  • 1106

end page

  • 1110

volume

  • 335

issue

  • 6072

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