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Gap-43, afgf, cck and alpha-cgrp and beta-cgrp in rat spinal motoneurons subjected to axotomy and or dorsal-root severance

Academic Article
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Overview

authors

  • Piehl, F.
  • Arvidsson, U.
  • Johnson, H.
  • Cullheim, S.
  • Dagerlind, A.
  • Ulfhake, B.
  • Cao, Y.
  • Elde, R.
  • Pettersson, R. F.
  • Terenius, Lars
  • Hokfelt, T.

publication date

  • October 1993

journal

  • European Journal of Neuroscience  Journal

abstract

  • The mRNA levels for growth-associated protein 43 (GAP-43), acidic fibroblast growth factor (aFGF), alpha- and beta-calcitonin gene-related peptide (CGRP), cholecystokinin (CCK) and choline acetyltransferase (ChAT) in rat lumbar spinal motoneurons were studied by in situ hybridization 1, 5 and 21 days and 20 weeks following unilateral peripheral nerve sectioning, ventral rhizotomy or dorsal rhizotomy. Furthermore, CGRP- and aFGF-like immunoreactivities in the ventral horn were studied using immunohistochemistry. One to 21 days after axotomy, GAP-43 and alpha-CGRP mRNAs increased in lesioned motoneurons, while the aFGF mRNA levels were marginally higher in motoneurons on the lesion side as compared to the control side. beta-CGRP, CCK and ChAT mRNA levels, on the other hand, decreased during the short-term response (1-21 days) to axotomy. After ventral rhizotomy, but not peripheral axotomy, there was complete disappearance of aFGF-like immunoreactivity in the ventral root proximal to the lesion. In animals subjected to long-term survival (20 weeks) after peripheral axotomy, the expression of all studied substances had returned to normal levels. Unilateral dorsal rhizotomy did not induce any substantial short- or long-term shifts in the cellular expression of the GAP-43, aFGF, CGRP and CCK peptides or their mRNAs in motoneurons of lesioned segments. These results indicate that peptides/proteins in motoneurons are expressed differentially after axotomy. Whereas alpha-CGRP and GAP-43 are up-regulated, CCK and beta-CGRP become down-regulated and aFGF is largely unaffected.

subject areas

  • Animals
  • Axons
  • Calcitonin Gene-Related Peptide
  • Cholecystokinin
  • Choline O-Acetyltransferase
  • Fibroblast Growth Factor 1
  • GAP-43 Protein
  • In Situ Hybridization
  • Male
  • Membrane Glycoproteins
  • Motor Neurons
  • Nerve Tissue Proteins
  • Neurofilament Proteins
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Sciatic Nerve
  • Spinal Cord
  • Spinal Nerve Roots
  • Time Factors
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Research

keywords

  • ACETYLCHOLINE
  • ACUTE AND CHRONIC AXOTOMY
  • DEAFFERENTATION
  • GROWTH-RELATED PROTEINS
  • IMMUNOHISTOCHEMISTRY
  • IN-SITU HYBRIDIZATION
  • PEPTIDES
  • SPINAL CORD
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Identity

International Standard Serial Number (ISSN)

  • 0953-816X

Digital Object Identifier (DOI)

  • 10.1111/j.1460-9568.1993.tb00918.x

PubMed ID

  • 7506107
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Additional Document Info

start page

  • 1321

end page

  • 1333

volume

  • 5

issue

  • 10

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