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Effects of a SARS-associated coronavirus vaccine in monkeys

Academic Article
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Overview

authors

  • Gao, W. T.
  • Tamin, A.
  • Soloff, A.
  • D'Aiuto, L.
  • Nwanegbo, E.
  • Robbins, Paul D.
  • Bellini, W. J.
  • Barratt-Boyes, S.
  • Gambotto, A.

publication date

  • December 2003

journal

  • Lancet  Journal

abstract

  • The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.

subject areas

  • Adenoviridae
  • Animals
  • Antibody Formation
  • Blotting, Western
  • Coronavirus
  • Disease Models, Animal
  • Humans
  • Macaca mulatta
  • Monkey Diseases
  • Nucleocapsid Proteins
  • SARS Virus
  • Severe Acute Respiratory Syndrome
  • T-Lymphocytes
  • Vaccination
  • Viral Envelope Proteins
  • Viral Vaccines
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Identity

International Standard Serial Number (ISSN)

  • 0140-6736

Digital Object Identifier (DOI)

  • 10.1016/s0140-6736(03)14962-8

PubMed ID

  • 14667748
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Additional Document Info

start page

  • 1895

end page

  • 1896

volume

  • 362

issue

  • 9399

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