recent publications

• academic article

  • Mercer, A. C., Gaj, T., Sirk, S. J., Lamb, B. M., Barbas III, C. F. Regulation of endogenous human gene expression by ligand-inducible TALE transcription factors ACS Synthetic Biology  2014 3:723-730  DOI:10.1021/sb400114p  PMID:24251925  PMCID:PMC4097969
  • Gaj, T., Liu, J., Anderson, K. E., Sirk, S. J., Barbas III, C. F. Protein delivery using Cys2-His2 zinc-finger domains ACS Chemical Biology  2014 9:1662-1667  DOI:10.1021/cb500282g  PMID:24936957  PMCID:PMC4519095
  • Sirk, S. J., Gaj, T., Jonsson, A., Mercer, A. C., Barbas III, C. F. Expanding the zinc-finger recombinase repertoire: directed evolution and mutational analysis of serine recombinase specificity determinants Nucleic Acids Research  2014 42:4755-4766  DOI:10.1093/nar/gkt1389  PMID:24452803  PMCID:PMC3985619
  • Liu, J., Gaj, T., Patterson, J. T., Sirk, S. J., Barbas III, C. F. Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering PLoS One  2014 9:e85755  DOI:10.1371/journal.pone.0085755  PMID:24465685  PMCID:PMC3896395
  • Gaj, T., Sirk, S. J., Barbas III, C. F. Expanding the scope of site-specific recombinases for genetic and metabolic engineering Biotechnology and Bioengineering  2014 111:1-15  DOI:10.1002/bit.25096  PMID:23982993  PMCID:PMC4097888
  • Gavrilyuk, J., Ban, H., Uehara, H., Sirk, S. J., Saye-Francisco, K., Cuevas, A., Zablowsky, E., Oza, A., Seaman, M. S., Burton, D. R., Barbas, C. F. Antibody conjugation approach enhances breadth and potency of neutralization of anti-HIV-1 antibodies and CD4-IgG Journal of Virology  2013 87:4985-4993  DOI:10.1128/jvi.03146-12  PMID:23427154  PMCID:PMC3624287
  • Gaj, T., Mercer, A. C., Sirk, S. J., Smith, H. L., Barbas III, C. F. A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells Nucleic Acids Research  2013 41:3937-3946  DOI:10.1093/nar/gkt071  PMID:23393187  PMCID:PMC3616721
  • Gaj, T., Guo, J., Kato, Y., Sirk, S. J., Barbas III, C. F. Targeted gene knockout by direct delivery of zinc-finger nuclease proteins Nature Methods  2012 9:805-7  DOI:10.1038/nmeth.2030  PMID:22751204  PMCID:PMC3424280