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Novel-approach to water proton relaxation in paramagnetic ion macromolecule complexes

Academic Article
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Overview

authors

  • Burton, Dennis
  • Dwek, R. A.
  • Forsen, S.
  • Karlstrom, G.

publication date

  • 1977

journal

  • Biochemistry  Journal

abstract

  • The use of the proton relaxation enhancement (PRE) technique in solutions of macromolecules and paramagnetic ions to obtain information on molecular motion and ion-hydration numbers can be hazardous with the possibility of large errors in the derived parameters. Here we describe a method based on solvent proton-deuteron comparative relaxation measurements at a limited number of different magnetic fields for obtaining, reasonably accurately, rapidly, and unambiguously, certain of the parameters normally obtained from a PRE study. In the non-immune rabbit immunoglobulin G(IgG)-Gd(iii) system, we are able to obtain values for the ion hydration number (q), the residence lifetime of water molecules in the hydration sphere of the ion (tauM) and a correlation time of 5 ns, which can be associated to a large part with a rotational correlation time (tauR) for the Fc portion (C-terminal half of heavy-chain dimer) of the IgG molecule. This shows that there is a rapid internal motion in the Fc region. The limitations and possible applications of the method are discussed.

subject areas

  • Binding Sites
  • Deuterium
  • Gadolinium
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Magnetic Resonance Spectroscopy
  • Models, Biological
  • Motion
  • Protein Conformation
  • Protons
  • Temperature
  • Water
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00621a014

PubMed ID

  • 836785
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Additional Document Info

start page

  • 250

end page

  • 254

volume

  • 16

issue

  • 2

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