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Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation

Academic Article
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Overview

authors

  • Leon, S.
  • Zhang, L.
  • McDonald, W. H.
  • Yates III, John
  • Cregg, J. M.
  • Subramani, S.

publication date

  • January 2006

journal

  • Journal of Cell Biology  Journal

abstract

  • We characterize the peroxin PpPex20p from Pichia pastoris and show its requirement for translocation of PTS2 cargoes into peroxisomes. PpPex20p docks at the peroxisomal membrane and translocates into peroxisomes. Its peroxisomal localization requires the docking peroxin Pex14p but not the peroxins Pex2p, Pex10p, and Pex12p, whose absence causes peroxisomal accumulation of Pex20p. Similarities between Pex5p and Pex20p were noted in their protein interactions and dynamics during import, and both contain a conserved NH2-terminal domain. In the absence of the E2-like Pex4p or the AAA proteins Pex1p and Pex6p, Pex20p is degraded via polyubiquitylation of residue K19, and the K19R mutation causes accumulation of Pex20p in peroxisome remnants. Finally, either interference with K48-branched polyubiquitylation or removal of the conserved NH2-terminal domain causes accumulation of Pex20p in peroxisomes, mimicking a defect in its recycling to the cytosol. Our data are consistent with a model in which Pex20p enters peroxisomes and recycles back to the cytosol in an ubiquitin-dependent manner.

subject areas

  • Cytosol
  • Endocytosis
  • Fungal Proteins
  • Gene Deletion
  • Models, Molecular
  • Peroxisomes
  • Pichia
  • Receptors, Cytoplasmic and Nuclear
  • Signal Transduction
  • Ubiquitin
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Identity

PubMed Central ID

  • PMC2063535

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.200508096

PubMed ID

  • 16390998
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Additional Document Info

start page

  • 67

end page

  • 78

volume

  • 172

issue

  • 1

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