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Mutagenesis by retroviral insertion in chemical mutagen-gene rated quasi-haploid mammalian cells

Academic Article
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Overview

authors

  • Kim, S. O.
  • Ha, S. D.
  • Lee, S.
  • Stanton, S.
  • Beutler, Bruce
  • Han, Jiahuai

publication date

  • April 2007

journal

  • Biotechniques  Journal

abstract

  • Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6-8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially designed retroviral vector that permits rapid identification of disrupted genes in each cell that bears a phenotype of interest. The utility of combined chemical and insertional mutagenesis is illustrated by the identification of novel host genes that are required for macrophage sensitivity to anthrax lethal factor.

subject areas

  • Animals
  • Base Sequence
  • Cell Line, Transformed
  • Haploidy
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutagens
  • Mutation
  • Phenotype
  • Retroviridae
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Identity

International Standard Serial Number (ISSN)

  • 0736-6205

Digital Object Identifier (DOI)

  • 10.2144/000112390

PubMed ID

  • 17489237
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Additional Document Info

start page

  • 493

end page

  • 501

volume

  • 42

issue

  • 4

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