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Mouse monoclonal antibodies to hepatitis b virus pres1 produced after immunization with recombinant pres1 peptide

Academic Article
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Overview

authors

  • Ryu, C. J.
  • Kim, Y. K.
  • Hur, H.
  • Kim, H. S.
  • Oh, J. M.
  • Kang, Young Jun
  • Hong, H. J.

publication date

  • April 2000

journal

  • Hybridoma  Journal

abstract

  • We have efficiently generated mouse monoclonal antibodies (MAbs), which bind specifically to amino acids 21-47 of the preS1 domain of hepatitis B virus (HBV) by immunizing mice with the preS1 peptide (amino acids, aa 1-56) conjugated to keyhole limpet hemocyanin. Hybridomas were screened by an indirect enzyme-linked immunosorbent assay (ELISA) using the purified preS1 peptide as a coated antigen. Eighteen positive hybridomas were selected and subjected to isotyping. Of these, 5 clones secreted immunoglobulin G (IgG) and 13 clones secreted IgM. Four (KR1, KR2, KR3, and KR4) of the 5 IgG MAbs bound to preS1 peptide (aa 21-47). Epitope mapping using bacterially expressed GST fusion proteins revealed that three clones (KR2, KR3, KR4) (IgG1, K) recognize aa 21-35, while KR1 (IgG2a, K) recognizes aa 35-47 of the preS1. These MAbs immunoprecipitated HBV particles, demonstrating that they bind to native HBV particles.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Epitope Mapping
  • Escherichia coli
  • Glutathione Transferase
  • Hepatitis B Surface Antigens
  • Hybridomas
  • Immunodominant Epitopes
  • Immunoglobulin Isotypes
  • Mice
  • Peptides
  • Precipitin Tests
  • Protein Precursors
  • Recombinant Fusion Proteins
  • Virion
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Identity

International Standard Serial Number (ISSN)

  • 0272-457X

Digital Object Identifier (DOI)

  • 10.1089/02724570050031248

PubMed ID

  • 10868800
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Additional Document Info

start page

  • 185

end page

  • 189

volume

  • 19

issue

  • 2

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