Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Ascl1 defines sequentially generated lineage-restricted neuronal and oligodendrocyte precursor cells in the spinal cord

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Battiste, J.
  • Helms, A. W.
  • Kim, E. J.
  • Savage, T. K.
  • Lagace, D. C.
  • Mandyam, Chitra
  • Eisch, A. J.
  • Miyoshi, G.
  • Johnson Jr., John

publication date

  • January 2007

journal

  • Development  Journal

abstract

  • The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricular zone cells in restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define the stage and neural lineages in the developing spinal cord that are derived from Ascl1-expressing cells. We find that Ascl1 is present in progenitors to both neurons and oligodendrocytes, but not astrocytes. Temporal control of the fate-mapping paradigm reveals rapid cell-cycle exit and differentiation of Ascl1-expressing cells. At embryonic day 11, Ascl1 identifies neuronal-restricted precursor cells that become dorsal horn neurons in the superficial laminae. By contrast, at embryonic day 16, Ascl1 identifies oligodendrocyte-restricted precursor cells that distribute throughout the spinal cord. These data demonstrate that sequentially generated Ascl1-expressing progenitors give rise first to dorsal horn interneurons and subsequently to late-born oligodendrocytes. Furthermore, Ascl1-null cells in the spinal cord have a diminished capacity to undergo neuronal differentiation, with a subset of these cells retaining characteristics of immature glial cells.

subject areas

  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA Primers
  • Embryonic Stem Cells
  • Female
  • Gene Expression Regulation, Developmental
  • Integrases
  • Interneurons
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Knockout
  • Mice, Transgenic
  • Neurons
  • Oligodendroglia
  • Pregnancy
  • Spinal Cord
  • Tamoxifen
scroll to property group menus

Research

keywords

  • Mash1 (Ascl1)
  • bHLH transcription factor
  • in vivo genetic fate mapping
  • mouse
  • spinal cord development
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0950-1991

Digital Object Identifier (DOI)

  • 10.1242/dev.02727

PubMed ID

  • 17166924
scroll to property group menus

Additional Document Info

start page

  • 285

end page

  • 293

volume

  • 134

issue

  • 2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support