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An aptamer that neutralizes r5 strains of hiv-1 binds to core residues of gp120 in the ccr5 binding site

Academic Article
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Overview

authors

  • Cohen, C.
  • Forzan, M.
  • Sproat, B.
  • Pantophlet, R.
  • McGowan, I.
  • Burton, Dennis
  • James, W.

publication date

  • November 2008

journal

  • Virology  Journal

abstract

  • We have previously isolated nucleic acid ligands (aptamers) that bind the surface envelope glycoprotein, gp120, of HIV-1, and neutralize infection of diverse sub-types of virus. Our earlier studies have identified the overall structure of one of these aptamers, B40, and have indicated that it binds to gp120 in a manner that competes with that of the HIV-1 coreceptor, CCR5, and select "CD4i" antibodies with epitopes overlapping this region. Here, we sought to map the B40 binding site on gp120 more precisely by analysing its interaction with a panel of alanine substitution mutants of gp120. Furthermore, we tested our hypothesis concerning the structure of the 40 nucleotide functional core of the aptamer by the solid-phase synthesis of truncated and chemically modified derivatives. The results confirm our structural predictions and demonstrate that aptamer B40 neutralizes a diverse range of HIV-1 isolates as a result of binding to relatively conserved residues on gp120 at the heart of the CCR5-binding site. These structural insights may provide the basis for the development of potential anti-viral agents with high specificity and robustness.

subject areas

  • Anti-HIV Agents
  • Aptamers, Nucleotide
  • Binding Sites
  • Cells, Cultured
  • HIV Envelope Protein gp120
  • HIV Infections
  • HIV-1
  • Humans
  • RNA
  • Receptors, CCR5
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Research

keywords

  • Aptamer
  • Coreceptor
  • HIV-1
  • Microbicide
  • Neutralization
  • gp120
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Identity

PubMed Central ID

  • PMC4380131

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2008.08.025

PubMed ID

  • 18799178
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Additional Document Info

start page

  • 46

end page

  • 54

volume

  • 381

issue

  • 1

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