Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Fischinger, P. J.
  • Thiel, H. J.
  • Ihle, J. N.
  • Lee, Jiing-Dwan
  • Elder, John

publication date

  • 1981

journal

  • Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences  Journal

abstract

  • X-irradiation of outbred Swiss mice resulted in the development of virus-free thymomas. When put in culture, a lymphoblastic cell line (NIXT) expressed neither particles nor infectious virus but supported the growth of pure ecotropic murine leukemia viruses (MuLVs) without generating any envelope recombinant (RM) MuLV in more than 20 months of culture. These cells did not support the growth of RM-MuLVs and completely excluded the entry of all RM-MuLV pseudotypes of murine sarcoma virus, suggesting specific viral interference. Radioimmunocompetition and immunofluorescence assays with broadly reactive anti-MuLV-p30 and -gp70 antisera were negative. However, in immunofluorescence with antisera specifically reactive against RM-MuLV gp70, about 5-20% of the population of parental cells or their clones were positive. NIXT cells treated with this antiserum bound protein A and exhibited complement-dependent cytotoxicity as assessed by several assays. NIXT cells could partially absorb neutralizing antibody specific for RM-MuLVs. Based on radioimmunoprecipitation tests, NIXT cells bore, on the cell surface, a glycosylated protein (gp70) reactive with RM subgroup as well as some group-specific anti-gp70 antisera. The glycoprotein was also found free in the supernates of NIXT cells. Using affinity chromatography, we determined the peptide pattern of the gp70 from NIXT cells to determine its structural relationship to gp70s of other MuLVs. NIXT gp70 was found to be highly related to class III endogenous xenotropic gp70s but, in addition, had peptide characteristics of RM-gp70s. Apparently, NIXT cells code for an unusual gp70 protein in the absence of other MuLV expression. The possible role of this glycoprotein in leukemogenesis is discussed.

subject areas

  • Animals
  • Cell Line
  • Epitopes
  • Glycoproteins
  • Immune Sera
  • Immunoassay
  • Leukemia Virus, Murine
  • Mice
  • Neoplasms, Experimental
  • Peptide Fragments
  • Recombination, Genetic
  • Thymoma
  • Thymus Neoplasms
  • Viral Proteins
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.78.3.1920

PubMed ID

  • 6165021
scroll to property group menus

Additional Document Info

start page

  • 1920

end page

  • 1924

volume

  • 78

issue

  • 3

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support