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Integrin alpha nu beta 1 is an adenovirus coreceptor

Academic Article
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Overview

authors

  • Li, E.
  • Brown, S. L.
  • Stupack, D. G.
  • Puente, X. S.
  • Cheresh, D. A.
  • Nemerow, Glen

publication date

  • June 2001

journal

  • Journal of Virology  Journal

abstract

  • The human embryonic kidney (HEK293) cell line, commonly used for recombinant adenovirus (Ad) propagation, does not express the Ad coreceptor alpha(v)beta3 or alpha(v)beta5 integrins, yet these cells are efficiently infected by Ad vectors. Here we demonstrate that Ad binds to HEK293 cells via the fiber receptor CAR and is subsequently internalized via interaction with integrin alpha(v)beta1. Function-blocking antibodies directed against alpha(v) or beta1, but not beta3, beta5, or alpha5, integrin subunits block Ad infection and viral endocytosis. Therefore, alpha(v)beta1 serves as a coreceptor for Ad infection, and the lack of beta3 and/or beta5 but the relatively high expression of alpha(v)beta1 integrins on certain tumor cell types may explain why these cells are readily transduced by Ad vectors.

subject areas

  • Adenoviridae
  • Capsid
  • Capsid Proteins
  • Cell Adhesion
  • Cell Line
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • Humans
  • Integrins
  • Receptors, Virus
  • Receptors, Vitronectin
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Identity

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.75.11.5405-5409.2001

PubMed ID

  • 11333925
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Additional Document Info

start page

  • 5405

end page

  • 5409

volume

  • 75

issue

  • 11

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