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Neural cell-adhesion molecules in rodent brains isolated by monoclonal-antibodies with cross-species reactivity

Academic Article
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Overview

authors

  • Chuong, C. M.
  • McClain, D. A.
  • Streit, P.
  • Edelman, Gerald

publication date

  • 1982

journal

  • Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences  Journal

abstract

  • Previous studies in this laboratory have led to the identification and purification of a chicken cell surface protein named "neural cell adhesion molecule" (N-CAM) that is involved in neural cell-cell and neurite-neurite interactions. In the present investigation, we have found that a similar molecule exists in the mouse and have confirmed that it is also present in rat neural tissue. A monoclonal antibody to chicken N-CAM that crossreacted with mouse and rat brains and an independently derived monoclonal antibody to mouse N-CAM were used to purify an antigen from perinatal mouse and rat brains. The purified neural antigen resembles chicken N-CAM in its ability to neutralize antibodies that inhibit neural cell aggregation and also in its biochemical properties including molecular weight, sialic acid content, amino acid composition, and autoconversion to a smaller polypeptide. Like chicken N-CAM, the murine molecule is found throughout the nervous system and over the entire neuronal cell surface. These results strongly suggest that the molecule is evolutionarily related to chicken N-CAM and prompt the hypothesis that cell adhesion involving N-CAM is a fundamental mechanism existing in nervous systems of different phylogenetic classes of animals.

subject areas

  • Aging
  • Amino Acids
  • Animals
  • Antibodies, Monoclonal
  • Antigens
  • Brain
  • Cell Adhesion Molecules
  • Cell Aggregation
  • Cross Reactions
  • Embryo, Mammalian
  • Female
  • Fluorescent Antibody Technique
  • Kinetics
  • Mice
  • Neurons
  • Pregnancy
  • Species Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.79.13.4234

PubMed ID

  • 7051008
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Additional Document Info

start page

  • 4234

end page

  • 4238

volume

  • 79

issue

  • 13

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