Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Analysis of the MCMV resistome by ENU mutagenesis

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Crozat, K.
  • Georgel, P.
  • Rutschmann, S.
  • Mann, N.
  • Du, X.
  • Hoebe, K.
  • Beutler, Bruce

publication date

  • May 2006

journal

  • Mammalian Genome  Journal

abstract

  • The mouse cytomegalovirus (MCMV) resistome is the set of host genes with nonredundant functions in resistance to MCMV infection. By screening 3,500 G(3) germline mutant mice ( approximately 1,750 gamete equivalents), we have identified eight transmissible mutations that create MCMV susceptibility in C57BL/6 mice. Among these, a mutation called Domino was noted to cause macrophage susceptibility to vesicular stomatitis virus (VSV) in vitro. This accessory phenotype was not corrected by type I interferon (IFN), which suggested a defect of the type I IFN pathway. Domino corresponds to a point mutation that alters the DNA binding domain of STAT1, leading to a defect of STAT1 activation. Identification of the Domino mutation demonstrates that an in vivo MCMV susceptibility screen is feasible and illustrates how it can provide insight into the resistome. Moreover, some mutations are far more deleterious than Domino in MCMV-infected mice, consistent with the interpretation that certain protein(s) unrelated to IFN production or signaling are more important than IFNs with regard to their net antiviral effects.

subject areas

  • Animals
  • Ethylnitrosourea
  • Germ-Line Mutation
  • Herpesviridae Infections
  • Immunity, Innate
  • Interferon Type I
  • Macrophages
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Muromegalovirus
  • Mutagenesis
  • Point Mutation
  • Rhabdoviridae Infections
  • STAT1 Transcription Factor
  • Transcription Factors
  • Vesicular stomatitis Indiana virus
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0938-8990

Digital Object Identifier (DOI)

  • 10.1007/s00335-005-0164-2

PubMed ID

  • 16688530
scroll to property group menus

Additional Document Info

start page

  • 398

end page

  • 406

volume

  • 17

issue

  • 5

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support