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Subregion- and cell type-restricted gene knockout in mouse brain

Academic Article
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Overview

authors

  • Tsien, J. Z.
  • Chen, D. F.
  • Gerber, D.
  • Tom, C.
  • Mercer, E. H.
  • Anderson, D. J.
  • Mayford, Mark
  • Kandel, E. R.
  • Tonegawa, S.

publication date

  • December 1996

journal

  • Cell  Journal

abstract

  • Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

subject areas

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Gene Expression
  • Gene Expression Regulation
  • Genetic Engineering
  • Gestational Age
  • Hippocampus
  • Integrases
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Recombination, Genetic
  • Sequence Deletion
  • Viral Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)81826-7

PubMed ID

  • 8980237
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Additional Document Info

start page

  • 1317

end page

  • 1326

volume

  • 87

issue

  • 7

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