Subregion- and cell type-restricted gene knockout in mouse brain

Academic Article

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Overview

authors

• Tsien, J. Z.
• Chen, D. F.
• Gerber, D.
• Tom, C.
• Mercer, E. H.
• Anderson, D. J.
• Mayford, Mark
• Kandel, E. R.
• Tonegawa, S.

publication date

• December 1996

journal

• Cell  Journal

abstract

• Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

subject areas

• Animals
• Calcium-Calmodulin-Dependent Protein Kinases
• Gene Expression
• Gene Expression Regulation
• Genetic Engineering
• Gestational Age
• Hippocampus
• Integrases
• Mice
• Mice, Knockout
• Mice, Transgenic
• Promoter Regions, Genetic
• RNA, Messenger
• Recombination, Genetic
• Sequence Deletion
• Viral Proteins

Identity

International Standard Serial Number (ISSN)

• 0092-8674

Digital Object Identifier (DOI)

• 10.1016/s0092-8674(00)81826-7

PubMed ID

• 8980237

Additional Document Info

start page

• 1317

end page

• 1326

volume

• 87

issue

• 7