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An aminoglycoside microarray platform for directly monitoring and studying antibiotic resistance

Academic Article
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Overview

authors

  • Disney, Matthew
  • Barrett, O. J.

publication date

  • October 2007

journal

  • Biochemistry  Journal

abstract

  • Antibiotic resistance is a major threat to human health. Since resistance to the aminoglycoside class of antibiotics is most commonly caused by enzymatic modification, we developed a high-throughput microarray platform for directly assaying resistance enzyme activity on aminoglycosides. After modification, the array can be hybridized with the therapeutic target, a bacterial rRNA A-site mimic, to study the effect that modification has on binding. Such studies will help identify important factors that contribute to high-affinity recognition of therapeutic targets and low-affinity recognition of and modification by resistance enzymes. This platform may also be useful for screening chemical libraries to discover new antibiotics that evade resistance.

subject areas

  • Adenosine Triphosphate
  • Aminoglycosides
  • Anti-Bacterial Agents
  • Binding Sites
  • Carbohydrate Sequence
  • Drug Resistance, Bacterial
  • Escherichia coli
  • Kanamycin
  • Kanamycin Kinase
  • Microbial Sensitivity Tests
  • Models, Chemical
  • Molecular Sequence Data
  • Molecular Structure
  • RNA, Ribosomal
  • Reproducibility of Results
  • Tobramycin
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi701071h

PubMed ID

  • 17867707
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Additional Document Info

start page

  • 11223

end page

  • 11230

volume

  • 46

issue

  • 40

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