Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Cellular requirements for antigen presentation in the induction of a thymus-independent antibody response in vitro

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Kirkland, T. N.
  • Sieckmann, D. G.
  • Longo, D. L.
  • Mosier, Donald

publication date

  • 1980

journal

  • Journal of Immunology  Journal

abstract

  • The ability of various cell populations to bind and present the thymus-independent antigen TNP-Ficoll to a responding cell population was assessed. The in vitro antibody response to TNP-Ficoll depends upon the presence of B lymphocytes and plastic-adherent accessory cells, but does not require T lymphocytes. Purified B cells were the most effective population in binding and presenting TNP-Ficoll, and adherent cells did not perform this function. Antigen binding and presentation was antigen specific and could be blocked with anti-mu antibody, but not by antibodies directed against other immunoglobulin classes. Spleen cells from mice genetically unresponsive to TNP-Ficoll (CBA/N X BALB/c F1 males) were equally effective as normal spleen cells in antigen binding and presentation. We conclude that the initial events in the induction of the antibody response involves antigen binding by B cells, and that subsequent activation of the subset of B cells that can respond to TNP-Ficoll proceeds either via B cell-B cell interaction or B cell-dependent transfer of antigen to macrophage-like cells.

subject areas

  • Animals
  • Antibody Formation
  • Antigens
  • B-Lymphocytes
  • Cell Adhesion
  • Cell Survival
  • Female
  • Ficoll
  • Immunity, Cellular
  • Immunoglobulin M
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Rabbits
  • T-Lymphocytes
  • Thymus Gland
  • Trinitrobenzenes
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 6965958
scroll to property group menus

Additional Document Info

start page

  • 1721

end page

  • 1726

volume

  • 124

issue

  • 4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support