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Blue fluorescent antibodies as reporters of steric accessibility in virus conjugates

Academic Article
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Overview

authors

  • Wang, Q.
  • Raja, K. S.
  • Janda, Kim
  • Lin, T. W.
  • Finn, M.G.

publication date

  • January 2003

journal

  • Bioconjugate Chemistry  Journal

abstract

  • Nonenveloped viruses provide the chemist with large, preassembled polyvalent protein scaffolds for modification. These structures are typically porous to small molecules but not to large ones. The solution-phase structures and reactivities of such assemblies may be substantially different than indicated by X-ray crystal structures. Here, the attachment of organic compounds to either the inside or outside surface of the cowpea mosaic virus (CPMV) coat protein was verified with an indicating antibody-antigen interaction. Antibody binding was subsequently blocked by the installation of poly(ethylene glycol) chains. These results typify the type of site-specific control that is available with CPMV and related virus building blocks.

subject areas

  • Antibodies, Viral
  • Antigen-Antibody Reactions
  • Capsid Proteins
  • Comovirus
  • Fluorescent Dyes
  • Models, Molecular
  • Organic Chemicals
  • Polyethylene Glycols
  • Viruses
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Identity

International Standard Serial Number (ISSN)

  • 1043-1802

Digital Object Identifier (DOI)

  • 10.1021/bc025587g

PubMed ID

  • 12526690
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Additional Document Info

start page

  • 38

end page

  • 43

volume

  • 14

issue

  • 1

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