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Peptides as antigens - importance of orientation

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Overview

authors

  • Dyrberg, T.
  • Oldstone, Michael

publication date

  • October 1986

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Factors known to be important in producing protein-reactive peptide antibodies include the accessibility of the region from which the peptide sequence is derived, the hydrophilic-phobic character of the sequence, and the length of the peptide. The data presented here indicate that the orientation of the peptide coupled to a carrier protein also influences the binding pattern of peptide antibodies. An octapeptide, representing a sequence from the alpha chain of the human acetylcholine receptor, was coupled either through an N- or C-terminal cysteine-glycine-glycine linker to a carrier protein and used to immunize rabbits. The resulting antisera reacted at comparable titers to the uncoupled immunizing peptides, but did not crossreact with the identical but opposite-linked peptide. Characterization of the binding to other homologous peptides showed that immunization with the N-terminal-linked peptide induced antibodies reactive specifically with the C-terminal amino acid(s). Immunization with the C-linked peptide resulted in antibodies reactive with a site of the peptide near the C-terminus.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antigens
  • Immunization
  • Peptides
  • Rabbits
  • Receptors, Cholinergic
  • Structure-Activity Relationship
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.164.4.1344

PubMed ID

  • 3760779
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Additional Document Info

start page

  • 1344

end page

  • 1349

volume

  • 164

issue

  • 4

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