Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Pharmacological evidence that a d2-receptor subtype mediates dopaminergic stimulation of prolactin secretion from the anterior-pituitary gland

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Burris, Thomas
  • Stringer, L. C.
  • Freeman, M. E.

publication date

  • August 1991

journal

  • Neuroendocrinology  Journal

abstract

  • The ability of low concentrations of dopamine (DA) to stimulate the secretion of prolactin (PRL) was examined in perifused or monolayer cultures of anterior pituitary cells. In cultures perifused with media containing 100 nM DA, changing the DA concentration to either 1 or 100 pM caused a significant dose-dependent stimulatory PRL secretory response within 6 min when compared to the PRL secretory response to removal of DA altogether. Picomolar concentrations of DA caused a biphasic PRL secretory response. This response is characterized by an immediate increase in the rate of PRL secretion similar to that seen when the cells were treated with 100 nM thyrotropin-releasing hormone followed by a decrease in the rate of PRL secretion to levels comparable to cells receiving media alone. In a monolayer culture system DA, at concentrations between 10 nM and 1.0 pM, caused significant stimulation of PRL secretion relative to media alone. Maximal stimulation occurred at nanomolar concentrations of DA (approximately 60% greater than control). Although the D2 agonists, bromocriptine and 2-(N-phenethyl-N-propyl)-amino-5-hydroxytetralin hydrochloride (PPHT) caused significant (p less than 0.05) inhibition of PRL secretion at nanomolar concentrations and above, neither had stimulatory activity. The D1 agonists, SKF 38393 and SKF 82958, had no effect on PRL secretion when tested at 0.1 pM to 1 microM. These data suggest that DA not only inhibits PRL secretion in vitro, but also stimulates PRL secretion at relatively low concentrations. Stimulation is mediated by a DA receptor which is neither recognized by D2 nor D1 agonists, suggesting a possible third DA receptor subtype.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Animals
  • Bromocriptine
  • Cells, Cultured
  • Dopamine
  • Dopamine Agents
  • Dopamine Antagonists
  • Female
  • Phenethylamines
  • Pituitary Gland, Anterior
  • Prolactin
  • Rats
  • Rats, Inbred Strains
  • Receptors, Dopamine
  • Receptors, Dopamine D2
  • Salicylamides
scroll to property group menus

Research

keywords

  • D1-RECEPTOR
  • D2-RECEPTOR SUBTYPE
  • HYPOTHALAMUS
  • PROLACTIN SECRETION, DOPAMINERGIC STIMULATION
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0028-3835

PubMed ID

  • 1685014
scroll to property group menus

Additional Document Info

start page

  • 175

end page

  • 183

volume

  • 54

issue

  • 2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support