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DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1

Academic Article
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Overview

authors

  • de Bruin, R. A. M.
  • Kalashnikova, T. I.
  • Aslanian, A.
  • Wohischlegel, J.
  • Chahwan, C.
  • Yates III, John
  • Russell, Paul
  • Wittenberg, Curt

publication date

  • August 2008

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • The cell cycle transcriptional program imposes order on events of the cell-cycle and is a target for signals that regulate cell-cycle progression, including checkpoints required to maintain genome integrity. Neither the mechanism nor functional significance of checkpoint regulation of the cell-cycle transcription program are established. We show that Nrm1, an MBF-specific transcriptional repressor acting at the transition from G(1) to S phase of the cell cycle, is at the nexus between the cell cycle transcriptional program and the DNA replication checkpoint in fission yeast. Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its dissociation from the MBF transcription factor. This leads to the expression of genes encoding components that function in DNA replication and repair pathways important for cell survival in response to arrested DNA replication.

subject areas

  • Checkpoint Kinase 2
  • DNA Repair
  • DNA Replication
  • DNA, Fungal
  • G1 Phase
  • Genome, Fungal
  • Phosphorylation
  • Protein-Serine-Threonine Kinases
  • Repressor Proteins
  • S Phase
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
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Research

keywords

  • ATR
  • CHK1
  • Cds1
  • Schizosaccharomyces pombe
  • cell cycle
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Identity

PubMed Central ID

  • PMC2516226

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0801106105

PubMed ID

  • 18682565
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Additional Document Info

start page

  • 11230

end page

  • 11235

volume

  • 105

issue

  • 32

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