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Migm-migd ratios on b-cells - mean migd expression exceeds migm by 10-fold on most splenic b-cells

Academic Article
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Overview

authors

  • Havran, Wendy
  • Digiusto, D. L.
  • Cambier, J. C.

publication date

  • 1984

journal

  • Journal of Immunology  Journal

abstract

  • The relative frequency of mIgM and mIgD molecules on B cell surfaces is important in determining, in large part, the isotype involvement in antigen binding and signal transduction. Although it is generally assumed that on most mature B cells, mIgM and mIgD occur in roughly equal quantities, no formal analysis of this question has been reported. In this report, we describe such an analysis based on the quantitation of anti-Fab or anti-kappa specific immunofluorescence of splenic B cells before or after capping with rabbit anti-IgD or anti-IgM antibodies or both. The results indicate that, whereas mean expression of IgD exceeds IgM on splenocytes by threefold, members of the major B cell subpopulation (60 to 70% of cells) express 10-fold more IgD than IgM.

subject areas

  • Animals
  • B-Lymphocytes
  • Fluorescent Antibody Technique
  • Immunoglobulin D
  • Immunoglobulin M
  • Immunoglobulin delta-Chains
  • Immunoglobulin mu-Chains
  • Immunologic Capping
  • Mice
  • Mice, Inbred Strains
  • Receptors, Antigen, B-Cell
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 6421926
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Additional Document Info

start page

  • 1712

end page

  • 1716

volume

  • 132

issue

  • 4

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