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H-1 NMR-study of conformation and molecular-dynamics of glycoprotein cow-colostrum trypsin-inhibitor

Academic Article
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Overview

authors

  • Wagner, G.
  • Wuthrich, Kurt
  • Tschesche, H.

publication date

  • 1978

journal

  • European Journal of Biochemistry  Journal

abstract

  • The glycoprotein cow colostrum trypsin inhibitor was investigated by high resolution 1H nuclear magnetic resonance (NMR) at 360 MHz and, on the basis of the NMR data, compared with the basic pancreatic trypsin inhibitor (Kunitz) from bovine tissue. Detailed studies of the chemical shifts and the exchange kinetics of the labile protons indicated extensive homologies between the spatial structures of the polypeptide chains in the two compounds. This was further corroborated by comparison of the NMR spectral features and the dynamic properties of the aromatic amino acid residues in the two inhibitors. It thus appears that the covalently attached carbohydrate moiety in the colostrum inhibitor has only very limited effects on the spatial structure of the protein part of the molecule. On the other hand, as evidenced by the NMR line widths, the carbohydrate attachment causes a pronounced restriction of the overall mobility of the molecule, indicating a sizeable increase of the average radius of gyration as compared to the basic pancreatic trypsin inhibitor. Possible spatial arrangements of the globular polypeptide and the carbohydrate moieties in the colostrum inhibitor, which would be compatible with the experimental observations, are discussed.

subject areas

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Colostrum
  • Female
  • Glycoproteins
  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Mathematics
  • Pregnancy
  • Protein Conformation
  • Trypsin Inhibitors
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Identity

International Standard Serial Number (ISSN)

  • 0014-2956

Digital Object Identifier (DOI)

  • 10.1111/j.1432-1033.1978.tb12285.x

PubMed ID

  • 658047
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Additional Document Info

start page

  • 67

end page

  • 76

volume

  • 86

issue

  • 1

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