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A mRNA variant encoding a soluble form of 4-1BB, a member of the murine NGf/TNF receptor family

Academic Article
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Overview

authors

  • Setareh, M.
  • Schwarz, H.
  • Lotz, Martin

publication date

  • October 1995

journal

  • Gene  Journal

abstract

  • The murine 4-1BB gene, encoding a member of the nerve growth factor/tumor necrosis factor (NGF/TNF) receptor family, is thought to be selectively expressed in T cells and is involved in the regulation of lymphocyte proliferation. We detected two forms of the 4-1BB mRNA by RT-PCR which were expressed in an activation-dependent pattern in splenocytes and thymocytes. cDNA sequencing showed that the smaller form was a mRNA splice variant lacking the transmembrane region (4-1BB delta TM), because of the deletion of exon 8. The two forms of mRNA are differentially expressed in murine T cells, macrophages, 3T3 fibroblasts and epitheloid cells. Northern blotting also identifies two forms of mRNA of 1.5 and 2.4 kb, and the cell-type-specific pattern correlated with the PCR results. These results identify a novel form of 4-1BB. This and the previously known membrane-associated form have a broad tissue distribution, suggesting a more diverse role in host defense.

subject areas

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Antigens, CD137
  • Base Sequence
  • Blotting, Northern
  • DNA Primers
  • Exons
  • Gene Expression
  • Genetic Variation
  • Lymphocyte Activation
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Polymerase Chain Reaction
  • Protein Sorting Signals
  • RNA, Messenger
  • Receptors, Nerve Growth Factor
  • Receptors, Tumor Necrosis Factor
  • T-Lymphocytes
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Research

keywords

  • NERVE GROWTH FACTOR
  • SOLUBLE RECEPTOR
  • TUMOR NECROSIS FACTOR RECEPTOR FAMILY
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Identity

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(95)00349-b

PubMed ID

  • 7590349
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Additional Document Info

start page

  • 311

end page

  • 315

volume

  • 164

issue

  • 2

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