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Control of the yeast-cell cycle is associated with assembly disassembly of the cdc28 protein-kinase complex

Academic Article
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Overview

authors

  • Wittenberg, Curt
  • Reed, Steven

publication date

  • September 1988

journal

  • Cell  Journal

abstract

  • The Saccharomyces cerevisiae gene CDC28 encodes a protein kinase required for progression from G1 to S phase in the cell cycle. We present evidence that the active form of the Cdc28 protein kinase is a complex of approximately 160 kd containing an endogenous substrate, p40, and possibly other polypeptides. This complex phosphorylates p40 and exogenous histone H1 in vitro. Cell cycle arrest during G1 results in inactivation of the protein kinase accompanied by the disassembly of the complex. Furthermore, assembly of the complex is regulated during the cell cycle, reaching a maximum during G1. Partial complexes thought to be intermediates in the assembly process phosphorylate histone H1 but not p40. Addition of soluble factors to these partial complexes in vitro restores p40 phosphorylation and causes the complex to increase to the mature size. A model is presented in which p40 phosphorylation is required during G1 for cells to initiate a new cell cycle.

subject areas

  • Cell Cycle
  • Chromatography, Gel
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Enzyme Activation
  • Fungal Proteins
  • Histones
  • Immunologic Techniques
  • Models, Biological
  • Phosphoproteins
  • Phosphorylation
  • Protein Kinases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Substrate Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(88)90121-3

PubMed ID

  • 3046752
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Additional Document Info

start page

  • 1061

end page

  • 1072

volume

  • 54

issue

  • 7

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