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Mammalian Sly1 regulates syntaxin 5 function in endoplasmic reticulum to Golgi transport

Academic Article
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Overview

authors

  • Dascher, C.
  • Balch, William E.

publication date

  • July 1996

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Members of the syntaxin gene family are components of protein complexes which regulate vesicle docking and/or fusion during transport of cargo through the secretory pathway of eukaryotic cells. We have previously demonstrated that syntaxin 5 is specifically required for endoplasmic reticulum to Golgi transport (Dascher, C., Matteson, J., and Balch, W. E.(1994) J. Biol. Chem. 269, 29363-29366). To extend these observations we have now cloned a protein from rat liver membranes which forms a native complex with syntaxin 5. We demonstrate that this protein is the mammalian homologue to yeast Sly1p, previously identified as a protein which genetically and biochemically interacts with the small GTPase Ypt1p and Sed5p, proteins involved in docking/fusion in the early secretory pathway of yeast. Using transient expression we find that overexpression of rat liver Sly1 (rSly1) can neutralize the dominant negative effects of excess syntaxin 5 on endoplasmic reticulum to Golgi transport. These results suggest that rSly1 functions to positively regulate syntaxin 5 function.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans
  • Carrier Proteins
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • DNA, Complementary
  • Endoplasmic Reticulum
  • Fungal Proteins
  • Golgi Apparatus
  • Humans
  • Immediate-Early Proteins
  • Liver
  • Mammals
  • Membrane Proteins
  • Microsomes, Liver
  • Molecular Sequence Data
  • Munc18 Proteins
  • Myocardium
  • Protein Processing, Post-Translational
  • Qa-SNARE Proteins
  • Rats
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 8663406
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Additional Document Info

start page

  • 15866

end page

  • 15869

volume

  • 271

issue

  • 27

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