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Isolation of human t-lymphocytes and b-lymphocytes by rosette formation with 2-aminoethylisothiouronium bromide (aet) - treated sheep red blood-cells and with monkey red blood-cells

Academic Article
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Overview

authors

  • Pellegrino, M. A.
  • Ferrone, S.
  • Theofilopoulos, Argyrios

publication date

  • 1976

journal

  • Journal of Immunological Methods  Journal

abstract

  • Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.

subject areas

  • Animals
  • B-Lymphocytes
  • Cell Separation
  • Centrifugation, Density Gradient
  • Haplorhini
  • Humans
  • Immune Adherence Reaction
  • Macaca
  • Sheep
  • T-Lymphocytes
  • beta-Aminoethyl Isothiourea
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Identity

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(76)90120-4

PubMed ID

  • 819587
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Additional Document Info

start page

  • 273

end page

  • 279

volume

  • 11

issue

  • 3-4

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