Isolation of human t-lymphocytes and b-lymphocytes by rosette formation with 2-aminoethylisothiouronium bromide (aet) - treated sheep red blood-cells and with monkey red blood-cells

Academic Article
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publication date

  • 1976

abstract

  • Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.