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Recombinant expression, biochemical-characterization, and biological-activities of the human MGSA-gro protein

Academic Article
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Overview

authors

  • Derynck, R.
  • Balentien, E.
  • Han, Jiahuai
  • Thomas, H. G.
  • Wen, D. Z.
  • Samantha, A. K.
  • Zachariae, C. O.
  • Griffin, Patrick
  • Brachmann, R.
  • Wong, W. L.
  • Matsushima, K.
  • Richmond, A.

publication date

  • November 1990

journal

  • Biochemistry  Journal

abstract

  • Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterization shows that the recombinant protein is identical with the natural protein and is devoid of posttranslational glycosylation, sulfation, and phosphorylation. The two C-terminal amino acids are proteolytically removed from the mature recombinant MGSA, indicating a length of 71 instead of the predicted 73 amino acids. The recombinant MGSA is mitogenically active on the Hs294T melanoma cells. The purified MGSA competes with interleukin 8 for binding to neutrophil receptors and exhibits neutrophil chemotactic activity equivalent to that of interleukin 8.

subject areas

  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • Cell Division
  • Cell Line
  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotaxis, Leukocyte
  • Cricetinae
  • Cricetulus
  • DNA
  • Fibroblasts
  • Genetic Vectors
  • Growth Substances
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Melanoma
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Neutrophils
  • Recombinant Fusion Proteins
  • Tumor Cells, Cultured
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00496a011

PubMed ID

  • 2271650
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Additional Document Info

start page

  • 10225

end page

  • 10233

volume

  • 29

issue

  • 44

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