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Coiled bodies in the nucleolus of breast cancer cells

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Overview

authors

  • Ochs, R. L.
  • Stein, T. W.
  • Tan, Eng

publication date

  • February 1994

journal

  • Journal of Cell Science  Journal

abstract

  • Coiled bodies are a special type of small round nuclear body, composed of coiled fibers and granules, especially prominent in the nucleoplasm of highly active cells (Brasch and Ochs (1992) Exp. Cell Res. 202, 211-223). Although no specific function has been assigned to coiled bodies, they contain spliceosome snRNAs and proteins, as well as the nucleolar U3 RNA-associated protein fibrillarin. In the present study, we have used antibodies to the coiled body-specific protein p80-coilin, together with double-label immunofluorescence, confocal microscopy and immunoelectron microscopy, to examine the distribution of coiled bodies in a number of different breast cancer cell lines. By immunofluorescence, all cell lines had prominent coiled bodies in the nucleoplasm and several cell lines appeared to have coiled bodies within the nucleolus itself. Double-label immunofluorescence and confocal laser scanning microscopy confirmed the nucleolar localization of coiled bodies. Besides containing p80-coilin, nucleoplasmic and nucleolar coiled bodies contained fibrillarin and Sm proteins. By conventional and immunoelectron microscopy, nucleolar coiled bodies appeared as discrete structures within the nucleolus in a number of different morphotypes, distinct from the normal nucleolar domains of granular component, dense fibrillar component, and fibrillar centers. While the significance of finding coiled bodies in the nucleolus of certain breast cancer cell lines is at present unknown, this represents the first report of coiled bodies and Sm staining in the nucleolus of mammalian cells.

subject areas

  • Autoantigens
  • Breast Neoplasms
  • Cell Nucleolus
  • Chromosomal Proteins, Non-Histone
  • Female
  • Humans
  • Inclusion Bodies
  • Microscopy, Immunoelectron
  • Nuclear Proteins
  • Ribonucleoproteins, Small Nuclear
  • Tumor Cells, Cultured
  • snRNP Core Proteins
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Research

keywords

  • BREAST CANCER
  • COILED BODY
  • NUCLEOLUS
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Identity

International Standard Serial Number (ISSN)

  • 0021-9533

PubMed ID

  • 8207070
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Additional Document Info

start page

  • 385

end page

  • 399

volume

  • 107

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