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Replication dynamics of the yeast genome

Academic Article
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Overview

authors

  • Raghuraman, M. K.
  • Winzeler, Elizabeth
  • Collingwood, D.
  • Hunt, S.
  • Wodicka, L.
  • Conway, A.
  • Lockhart, D. J.
  • Davis, R. W.
  • Brewer, B. J.
  • Fangman, W. L.

publication date

  • 2001

journal

  • Science  Journal

abstract

  • Oligonucleotide microarrays were used to map the detailed topography of chromosome replication in the budding yeast Saccharomyces cerevisiae. The times of replication of thousands of sites across the genome were determined by hybridizing replicated and unreplicated DNAs, isolated at different times in S phase, to the microarrays. Origin activations take place continuously throughout S phase but with most firings near mid-S phase. Rates of replication fork movement vary greatly from region to region in the genome. The two ends of each of the 16 chromosomes are highly correlated in their times of replication. This microarray approach is readily applicable to other organisms, including humans.

subject areas

  • Algorithms
  • Base Sequence
  • Centromere
  • Chromosomes, Fungal
  • DNA Replication
  • DNA, Fungal
  • DNA, Intergenic
  • Fourier Analysis
  • Genome, Fungal
  • Kinetics
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis
  • Replication Origin
  • S Phase
  • Saccharomyces cerevisiae
  • Telomere
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.294.5540.115

PubMed ID

  • 11588253
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Additional Document Info

start page

  • 115

end page

  • 121

volume

  • 294

issue

  • 5540

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