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Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization

Academic Article
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Overview

authors

  • Wang, H.
  • Griffiths, M. N.
  • Burton, Dennis
  • Ghazal, Peter

publication date

  • January 2000

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • We have compared the kinetics of antibody responses in conventional and dendritic cell-targeted immunization by using a model antigen in mice. Targeting was achieved by linking the reporter antigen (polyclonal goat anti-hamster antibody) to N418, a hamster mAb that binds to the CD11c molecule on the surface of murine dendritic cells. Intradermal injection of submicrogram quantities of goat anti-hamster antibody complexed to mAb N418 elicited goat antibody-specific serum IgG in mice. Antigen-specific IgG titers were detectable by day 5, with titers that ranged from 1:1000 to 1:100,000 by day 7. In contrast, when the goat antigen was injected alone or in the presence of a hamster antibody control to form nontargeted complexes, goat-specific serum IgG was undetectable at day 7. Additional control experiments showed that the interaction between the model antigen and mAb N418 is required for amplification of the serum antibody response. These studies demonstrate that a single-step, facilitated-delivery of small amounts of protein antigen to dendritic cells in vivo can give very rapid and high antibody responses. The approach may be particularly useful for vaccination immediately before or just after exposure to a pathogen and may enhance the utility of subunit antigens as immunogens.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antibody Formation
  • Antibody Specificity
  • Cricetinae
  • Dendritic Cells
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Drug Synergism
  • Goats
  • Immunization
  • Immunoglobulin G
  • Injections, Intradermal
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.97.2.847

PubMed ID

  • 10639168
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Additional Document Info

start page

  • 847

end page

  • 852

volume

  • 97

issue

  • 2

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