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Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers

Academic Article
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Overview

authors

  • Chang, C. F.
  • Pan, J. F.
  • Wu, I. L.
  • Wong, Chi-Huey
  • Lin, Chun-Hung

publication date

  • 2011

journal

  • Glycobiology  Journal

abstract

  • Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.

subject areas

  • Acrylic Resins
  • Antibodies
  • Humans
  • Lectins
  • Microarray Analysis
  • Photosensitizing Agents
  • Polysaccharides
  • Protein Binding
  • Proteins
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Research

keywords

  • carbohydrates
  • lectins
  • microarray
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Identity

International Standard Serial Number (ISSN)

  • 0959-6658

Digital Object Identifier (DOI)

  • 10.1093/glycob/cwr021

PubMed ID

  • 21325337
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Additional Document Info

start page

  • 895

end page

  • 902

volume

  • 21

issue

  • 7

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