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Evidence that a major determinant for the identity of a transfer RNA is conserved in evolution

Academic Article
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Overview

authors

  • Hou, Y. M.
  • Schimmel, Paul

publication date

  • August 1989

journal

  • Biochemistry  Journal

abstract

  • We observed recently that a single G3.U70 base pair in the amino acid acceptor stem of an Escherichia coli alanine tRNA is a major determinant for its identity. Inspection of tRNA sequences shows that G3.U70 is unique to alanine in E. coli and is present in eucaryotic cytoplasmic alanine tRNAs. We show here that single nucleotide changes of G3.U70 to A3.U70 or to G3.C70 eliminate in vitro aminoacylation of an insect and of a human alanine tRNA by the respective homologous synthetase. Compared to the influence of G3.U70, other sequence variations in tRNAAla have a relatively small effect on aminoacylation by the insect and human enzymes. In addition, while these eucaryotic tRNAs have nucleotide differences from E. coli alanine tRNA, they are heterologously charged only with alanine when expressed in E. coli. The results indicate a functional role for G3.U70 that is conserved in evolution. They also suggest that the sequence differences between E. coli and the eucaryotic alanine tRNAs at sites other than the conserved G3.U70 do not create major determinants for recognition by any other bacterial enzyme.

subject areas

  • Alanine-tRNA Ligase
  • Animals
  • Base Composition
  • Base Sequence
  • Biological Evolution
  • Bombyx
  • Escherichia coli
  • Humans
  • Nucleic Acid Conformation
  • RNA, Transfer, Ala
  • RNA, Transfer, Amino Acid-Specific
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi00443a003

PubMed ID

  • 2684266
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Additional Document Info

start page

  • 6800

end page

  • 6804

volume

  • 28

issue

  • 17

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