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Old world arenavirus infection interferes with the expression of functional alpha-dystroglycan in the host cell

Academic Article
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Overview

authors

  • Rojek, J. M.
  • Campbell, K. P.
  • Oldstone, Michael
  • Kunz, S.

publication date

  • November 2007

journal

  • Molecular Biology of the Cell  Journal

abstract

  • alpha-Dystroglycan (alpha-DG) is an important cellular receptor for extracellular matrix (ECM) proteins as well as the Old World arenaviruses lymphocytic choriomeningitis virus (LCMV) and the human pathogenic Lassa fever virus (LFV). Specific O-glycosylation of alpha-DG is critical for its function as receptor for ECM proteins and arenaviruses. Here, we investigated the impact of arenavirus infection on alpha-DG expression. Infection with an immunosuppressive LCMV isolate caused a marked reduction in expression of functional alpha-DG without affecting biosynthesis of DG core protein or global cell surface glycoprotein expression. The effect was caused by the viral glycoprotein (GP), and it critically depended on alpha-DG binding affinity and GP maturation. An equivalent effect was observed with LFVGP. Viral GP was found to associate with a complex between DG and the glycosyltransferase LARGE in the Golgi. Overexpression of LARGE restored functional alpha-DG expression in infected cells. We provide evidence that virus-induced down-modulation of functional alpha-DG perturbs DG-mediated assembly of laminin at the cell surface, affecting normal cell-matrix interactions.

subject areas

  • Animals
  • Arenaviruses, Old World
  • Cell Line
  • Cercopithecus aethiops
  • Dystroglycans
  • Gene Expression Regulation
  • Glycosylation
  • Glycosyltransferases
  • Humans
  • Laminin
  • Mice
  • Protein Binding
  • Transcription, Genetic
  • Viral Proteins
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Identity

PubMed Central ID

  • PMC2043543

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.E07-04-0374

PubMed ID

  • 17761532
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Additional Document Info

start page

  • 4493

end page

  • 4507

volume

  • 18

issue

  • 11

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