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The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA

Academic Article
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Overview

authors

  • Sim, S.
  • Yao, J.
  • Weinberg, D. E.
  • Niessen, Sherry
  • Yates III, John
  • Wolin, S. L.

publication date

  • January 2012

journal

  • RNA-a Publication of the RNA Society  Journal

abstract

  • The Ro 60-kDa autoantigen, a ring-shaped RNA-binding protein, traffics between the nucleus and cytoplasm in vertebrate cells. In some vertebrate nuclei, Ro binds misfolded noncoding RNAs and may function in quality control. In the cytoplasm, Ro binds noncoding RNAs called Y RNAs. Y RNA binding blocks a nuclear accumulation signal, retaining Ro in the cytoplasm. Following UV irradiation, this signal becomes accessible, allowing Ro to accumulate in nuclei. To investigate how other cellular components influence the function and subcellular location of Ro, we identified several proteins that copurify with the mouse Ro protein. Here, we report that the zipcode-binding protein ZBP1 influences the subcellular localization of both Ro and the Y3 RNA. Binding of ZBP1 to the Ro/Y3 complex increases after UV irradiation and requires the Y3 RNA. Despite the lack of an identifiable CRM1-dependent export signal, nuclear export of Ro is sensitive to the CRM1 inhibitor leptomycin B. In agreement with a previous report, we find that ZBP1 export is partly dependent on CRM1. Both Ro and Y3 RNA accumulate in nuclei when ZBP1 is depleted. Our data indicate that ZBP1 may function as an adapter to export the Ro/Y3 RNA complex from nuclei.

subject areas

  • Active Transport, Cell Nucleus
  • Animals
  • Autoantigens
  • Cell Line
  • Cell Nucleus
  • Fatty Acids, Unsaturated
  • Glycoproteins
  • Karyopherins
  • Mice
  • RNA, Small Cytoplasmic
  • RNA, Untranslated
  • Receptors, Cytoplasmic and Nuclear
  • Ribonucleoproteins
  • Ultraviolet Rays
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Research

keywords

  • Ro autoantigen
  • noncoding RNA
  • nuclear transport
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Identity

PubMed Central ID

  • PMC3261732

International Standard Serial Number (ISSN)

  • 1355-8382

Digital Object Identifier (DOI)

  • 10.1261/rna.029207.111

PubMed ID

  • 22114317
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Additional Document Info

start page

  • 100

end page

  • 110

volume

  • 18

issue

  • 1

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