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Influenza-c virus uses 9-o-acetyl-n-acetylneuraminic acid as a high-affinity receptor determinant for attachment to cells

Academic Article
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Overview

authors

  • Rogers, G. N.
  • Herrler, G.
  • Paulson, James
  • Klenk, H. D.

publication date

  • May 1986

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Identification of the receptor-destroying enzyme of influenza C virus as a specific neuraminate O-acetylesterase has suggested that 9-O-acetyl-N-acetylneuraminic acid is an essential component of the cell surface receptor of influenza C virus (Herrler, G., Rott, R., Klenk, H.-D., Muller, H.-P., Shukla, A. K., and Schauer, R. (1985) EMBO (Eur. Mol. Biol. Organ.) J. 4, 1503-1506). In this report, three common sialic acids, N-acetylneuraminic acid (NeuAc), N-glycollylneuraminic acid (NeuGc), and 9-O-acetyl-N-acetylneuraminic acid (9-O-Ac-NeuAc) were compared for their ability to mediate attachment of influenza A, B, and C viruses to cells. Human asialoerythrocytes were resialylated to contain the three sialic acids in defined sequence on glycoprotein carbohydrate groups using purified sialyltransferases and corresponding CMP-sialic acid donor substrates. While influenza C virus failed to agglutinate native cells or resialylated cells containing NeuAc and NeuGc, resialylated cells containing 9-O-Ac-NeuAc in three different sialyloligosaccharide sequences were agglutinated in high titer. In contrast, most representative influenza A and B viruses examined preferentially agglutinated cells containing NeuAc and NeuGc and failed to agglutinate cells containing 9-O-Ac-NeuAc. Cells containing 9-O-Ac-NeuAc were sensitive to the action of influenza C virus neuraminate O-acetylesterase which converts 9-O-Ac-NeuAc to NeuAc. This treatment abolished agglutination by influenza C while making the cells agglutinable by several influenza A and B viruses. Finally, the ability of influenza C virus to agglutinate the erythrocytes of various species correlated with the presence of 9-O-Ac-NeuAc. The results provide direct evidence that influenza C virus utilizes 9-O-acetyl-N-acetylneuraminic acid as the primary receptor determinant for attachment to cell surface receptors.

subject areas

  • Animals
  • Chick Embryo
  • Hemagglutination
  • Humans
  • Influenza A virus
  • Influenza B virus
  • Influenzavirus C
  • Orthomyxoviridae
  • Receptors, Virus
  • Sialic Acids
  • Species Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 3700379
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Additional Document Info

start page

  • 5947

end page

  • 5951

volume

  • 261

issue

  • 13

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