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Nmr characterization of a ph-dependent equilibrium between two folded solution conformations of the pheromone-binding protein from bombyx mori

Academic Article
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Overview

authors

  • Damberger, F.
  • Nikonova, L.
  • Horst, R.
  • Peng, G. H.
  • Leal, W. S.
  • Wuthrich, Kurt

publication date

  • May 2000

journal

  • Protein Science  Journal

abstract

  • NMR spectroscopic changes as a function of pH in solutions of the pheromone-binding protein of Bombyx mori (BmPBP) show that BmPBP undergoes a conformational transition between pH 4.9 and 6.0. At pH below 4.9 there is a single "acid form" (A), and a homogeneous "basic form" (B) exists at pH above 6.0. Between pH 5 and 6, BmPBP exists as a mixture of A and B in slow exchange on the NMR chemical shift time scale, with the transition midpoint at pH 5.4. The form B has a well-dispersed NMR spectrum, indicating that it represents a more structured, "closed" conformation than form A, which has a significantly narrower chemical shift dispersion. Conformational transitions of the kind observed here may explain heterogeneity reported for a variety of odorant-binding proteins, and it will be of interest to further investigate possible correlations with pH-dependent regulation of ligand binding and release in the biological function of this class of proteins.

subject areas

  • Animals
  • Bombyx
  • Carrier Proteins
  • Chromatography, High Pressure Liquid
  • Circular Dichroism
  • Escherichia coli
  • Hydrogen-Ion Concentration
  • Insect Proteins
  • Magnetic Resonance Spectroscopy
  • Protein Conformation
  • Protein Isoforms
  • Recombinant Proteins
  • Software
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Research

keywords

  • NMR monitored pH titration
  • insect odorant-binding protein
  • pH-dependent conformation
  • pheromone-binding protein
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Identity

PubMed Central ID

  • PMC2144629

International Standard Serial Number (ISSN)

  • 0961-8368

Digital Object Identifier (DOI)

  • 10.1110/ps.9.5.1038

PubMed ID

  • 10850815
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Additional Document Info

start page

  • 1038

end page

  • 1041

volume

  • 9

issue

  • 5

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