related to degree

• Xie, Jianming, Ph.D. in Chemistry, Scripps Research 2001 - 2006

authors

• Schultz, K. C.
• Supekova, L.
• Ryu, Y. H.
• Xie, Jianming
• Perera, R.
• Schultz, Peter

publication date

• 2006

journal

• Journal of the American Chemical Society  Journal

abstract

• An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.

subject areas

• Models, Molecular
• Molecular Probes
• Spectroscopy, Fourier Transform Infrared

International Standard Serial Number (ISSN)

• 0002-7863

Digital Object Identifier (DOI)

• 10.1021/ja0636690

PubMed ID

• 17061854

start page

• 13984

end page

• 13985

volume

• 128

issue

• 43