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A genetically encoded infrared probe

Academic Article
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Overview

related to degree

  • Xie, Jianming, Ph.D. in Chemistry, Scripps Research 2001 - 2006

authors

  • Schultz, K. C.
  • Supekova, L.
  • Ryu, Y. H.
  • Xie, Jianming
  • Perera, R.
  • Schultz, Peter

publication date

  • November 2006

journal

  • Journal of the American Chemical Society  Journal

abstract

  • An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes.

subject areas

  • Models, Molecular
  • Molecular Probes
  • Spectroscopy, Fourier Transform Infrared
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Identity

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja0636690

PubMed ID

  • 17061854
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Additional Document Info

start page

  • 13984

end page

  • 13985

volume

  • 128

issue

  • 43

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