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A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans

Academic Article
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Overview

authors

  • Audhya, A.
  • Hyndman, F.
  • McLeod, I. X.
  • Maddox, A. S.
  • Yates III, John
  • Desai, A.
  • Oegema, K.

publication date

  • October 2005

journal

  • Journal of Cell Biology  Journal

abstract

  • Cytokinesis completes cell division and partitions the contents of one cell to the two daughter cells. Here we characterize CAR-1, a predicted RNA binding protein that is implicated in cytokinesis. CAR-1 localizes to germline-specific RNA-containing particles and copurifies with the essential RNA helicase, CGH-1, in an RNA-dependent fashion. The atypical Sm domain of CAR-1, which directly binds RNA, is dispensable for CAR-1 localization, but is critical for its function. Inhibition of CAR-1 by RNA-mediated depletion or mutation results in a specific defect in embryonic cytokinesis. This cytokinesis failure likely results from an anaphase spindle defect in which interzonal microtubule bundles that recruit Aurora B kinase and the kinesin, ZEN-4, fail to form between the separating chromosomes. Depletion of CGH-1 results in sterility, but partially depleted worms produce embryos that exhibit the CAR-1-depletion phenotype. Cumulatively, our results suggest that CAR-1 functions with CGH-1 to regulate a specific set of maternally loaded RNAs that is required for anaphase spindle structure and cytokinesis.

subject areas

  • Anaphase
  • Animals
  • Aurora Kinase B
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Cell Division
  • Kinesin
  • Protein-Serine-Threonine Kinases
  • RNA Interference
  • RNA Nucleotidyltransferases
  • RNA-Binding Proteins
  • Spindle Apparatus
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Identity

PubMed Central ID

  • PMC2171198

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1082/jcb.200506124

PubMed ID

  • 16247027
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Additional Document Info

start page

  • 267

end page

  • 279

volume

  • 171

issue

  • 2

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