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A prokaryote and human tRNA synthetase provide an essential RNA splicing function in yeast mitochondria

Academic Article
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Overview

authors

  • Houman, F.
  • Rho, S. B.
  • Zhang, J. S.
  • Shen, X. Y.
  • Wang, C. C.
  • Schimmel, Paul
  • Martinis, S. A.

publication date

  • 2000

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Mitochondrial leucyl-tRNA synthetase (LeuRS) in the yeast Saccharomyces cerevisiae provides two essential functions. In addition to aminoacylation, LeuRS functions in RNA splicing. The details of how it came to act in splicing are not known. Here we show that Mycobacterium tuberculosis and human mitochondrial LeuRSs can substitute in splicing for the S. cerevisiae mitochondrial LeuRS. Mutations of yeast mitochondrial LeuRS that had previously been shown to abolish splicing activity also eliminate splicing by the M. tuberculosis enzyme. These results suggest the role of LeuRS in splicing in yeast mitochondria results from features of the enzyme that are broadly conserved in evolution. These features are not likely to be designed for splicing per se, but instead have been adopted in yeast for that purpose.

subject areas

  • Amino Acid Sequence
  • Cloning, Molecular
  • Genetic Complementation Test
  • Humans
  • Introns
  • Leucine-tRNA Ligase
  • Molecular Sequence Data
  • Mycobacterium tuberculosis
  • RNA Splicing
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae
  • Sequence Homology, Amino Acid
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Identity

PubMed Central ID

  • PMC17646

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.240465597

PubMed ID

  • 11087829
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Additional Document Info

start page

  • 13743

end page

  • 13748

volume

  • 97

issue

  • 25

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