Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Encapsulation of lymphocyte DNA by vesicular stomatitis virus

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Kingsbury, D. T.
  • Lerner, Richard

publication date

  • 1974

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Vesicular stomatitis virus, grown on the WIL(2)-3A line of continuously growing human lymphocytes, contains DNA in addition to the viral RNA. By contrast, virus grown on BHK 21-C13 fibroblasts has no detectable DNA. The virus-associated DNA is found in both the B and T particles of the virus and is resistant to deoxyribonuclease. The DNA is intimately associated with the virus and appears to be incorporated into the viral ribonucleoprotein core structure produced by treatment of the virus with Nonidet P40 follow edby CsCl isopycnic banding. The virus-associated DNA has an isopycnic density of 1.699 g x cm(-) (-3) in CsCl, identical to that of human DNA. The average molecular weight of the DNA molecules associated with the virus is 9.0 x 10(5), as determined by velocity sedimentation in sucrose density gradients and studies of its contour length in the electron microscope. DNA.DNA reassociation kinetics of this DNA demonstrate that the DNA is of host origin and rules out the possibility that it originates from contaminating microorganisms or mitochondria. The analytical complexity of the virus-associated DNA shows that 50% of the virus-associated DNA sequences are homologous to the repeated DNA sequences of human DNA and the remaining 50% are homologous to human unique sequences. On the average, there is one molecule of DNA for each four virion particles.

subject areas

  • Animals
  • Base Sequence
  • Carbon Radioisotopes
  • Cell Line
  • Centrifugation, Density Gradient
  • Chemical Phenomena
  • Chemistry
  • Chromatography
  • Clone Cells
  • Cricetinae
  • DNA
  • DNA-Directed RNA Polymerases
  • Fibroblasts
  • Humans
  • Kidney
  • Lymphocytes
  • Microscopy, Electron
  • RNA-Directed DNA Polymerase
  • Recombination, Genetic
  • Thymidine
  • Uridine
  • Vesicular stomatitis Indiana virus
  • Virus Cultivation
scroll to property group menus

Identity

PubMed Central ID

  • PMC388317

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.71.5.1753

PubMed ID

  • 4134759
scroll to property group menus

Additional Document Info

start page

  • 1753

end page

  • 1757

volume

  • 71

issue

  • 5

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support