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Measles-virus inhibits mitogen-induced t-cell proliferation but does not directly perturb the t-cell activation process inside the cell

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Overview

authors

  • Yanagi, Y.
  • Cubitt, B. A.
  • Oldstone, Michael

publication date

  • 1992

journal

  • Virology  Journal

abstract

  • Measles virus (MV) inhibits lymphocyte function in patients, as well as in cells infected in vitro. The proliferation of phytohemagglutinin-stimulated T lymphocytes is suppressed by in vitro MV infection, as shown by the diminished incorporation of [3H]thymidine into DNA and the reduced frequency of cells in the S phase of the cell cycle, as compared with mock-infected cells. MV infection itself, however, does not completely block DNA synthesis in infected cells, because infected T cells expressing MV antigens on the cell surface, isolated by fluorescence-activated cell sorter, could still proliferate. Northern blot analysis indicated that the expression of genes induced during T cell activation, such as those encoding interleukin 2 (IL-2), c-myc, IL-2 receptor, IL-6, c-myb, and cdc-2, was not significantly suppressed in MV-infected cells, suggesting that MV does not interfere with the T cell activation process. When anti-MV serum or carbobenzoxy-D-Phe-L-Phe-Gly, a synthetic oligopeptide known to inhibit MV-induced fusion, was added 24 hr after infection, the inhibition of T cell proliferation was reversed in a dose-dependent manner. From these results we propose a model for the inhibition of T cell proliferation by MV; MV glycoproteins expressed on the cell surface of infected cells interact with the MV receptor or other molecules on the cell membrane of adjacent T cells, which in turn affects the proliferation of those T cells.

subject areas

  • Antiviral Agents
  • Blotting, Northern
  • Cell Division
  • DNA Replication
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Gene Expression Regulation
  • Immune Sera
  • Immune Tolerance
  • Lymphocyte Activation
  • Measles virus
  • Oligopeptides
  • Phytohemagglutinins
  • T-Lymphocytes
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Identity

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(92)90316-h

PubMed ID

  • 1736530
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Additional Document Info

start page

  • 280

end page

  • 289

volume

  • 187

issue

  • 1

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