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Structural and biophysical insights into the ligand-free Pitx2 homeodomain and a ring dermoid of the cornea inducing homeodomain mutant

Academic Article
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Overview

authors

  • Doerdelmann, T.
  • Kojetin, Douglas
  • Baird-Titus, J. M.
  • Solt, Laura A.
  • Burris, Thomas
  • Rance, M.

publication date

  • January 2012

journal

  • Biochemistry  Journal

abstract

  • The homeodomain-containing transcription factor Pitx2 (pituitary homeobox protein 2) is present in many developing embryonic tissues, including the heart. Its homeodomain is responsible for the recognition and binding to target DNA sequences and thus constitutes a major functional unit in the Pitx2 protein. Nuclear magnetic resonance techniques were employed to determine the solution structure of the native Pitx2 homeodomain and a R24H mutant that causes autosomal dominantly inherited ring dermoid of the cornea syndrome. The structures reveal that both isoforms possess the canonical homeodomain fold. However, the R24H mutation results in a 2-fold increase in DNA binding affinity and a 5 °C decrease in thermal stability, while changing the dynamic environment of the homeodomain only locally. When introduced into full-length Pitx2c, the mutation results in an only 25% loss of transactivation activity. Our data correlate well with clinical observations suggesting a milder deficiency for the R24H mutation compared to those of other Pitx2 homeodomain mutations.
  • The homeodomain-containing transcription factor Pitx2 (pituitary homeobox protein 2) is present in many developing embryonic tissues, including the heart. Its homeodomain is responsible for the recognition and binding to target DNA sequences and thus constitutes a major functional unit in the Pitx2 protein. Nuclear magnetic resonance techniques were employed to determine the solution structure of the native Pitx2 homeodomain and a R24H mutant that causes autosomal dominantly inherited ring dermoid of the cornea syndrome. The structures reveal that both isoforms possess the canonical homeodomain fold. However, the R24H mutation results in a 2-fold increase in DNA binding affinity and a 5 �C decrease in thermal stability, while changing the dynamic environment of the homeodomain only locally. When introduced into full-length Pitx2c, the mutation results in an only 25% loss of transactivation activity. Our data correlate well with clinical observations suggesting a milder deficiency for the R24H mutation compared to those of other Pitx2 homeodomain mutations.

subject areas

  • Amino Acid Sequence
  • Biophysical Processes
  • Conjunctival Diseases
  • Corneal Diseases
  • DNA
  • Dermoid Cyst
  • Genes, Reporter
  • Histidine
  • Homeodomain Proteins
  • Humans
  • Hydrogen-Ion Concentration
  • Ligands
  • Luciferases
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Stability
  • Protein Structure, Tertiary
  • Solutions
  • Thermodynamics
  • Transcription Factors
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Identity

PubMed Central ID

  • PMC3264736

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi201639x

PubMed ID

  • 22224469
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Additional Document Info

start page

  • 665

end page

  • 676

volume

  • 51

issue

  • 2

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