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Genetic susceptibility to silver-induced anti-fibrillarin autoantibodies in mice

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Overview

authors

  • Hultman, P.
  • Ganowiak, K.
  • Turley, S. J.
  • Pollard, Kenneth Michael

publication date

  • December 1995

journal

  • Clinical Immunology and Immunopathology  Journal

abstract

  • Similar to mercuric chloride, silver nitrate has recently been shown to induce IgG autoantibodies targeting the nucleolar 34-kDa protein fibrillarin i SJL (H-2s) mice. In the present study we show that the autoimmunogenic effect of silver is dependent on intact T-cell function since SJL/N mice homozygous for the nude mutation (athymic), in contrast to the functionally T-cell-intact SJL/N-nu/+ littermates, did not develop anti-nucleolar/anti-fibrillarin autoantibodies (ANoA/AFA). The genetic susceptibility for silver-induced AFA was localized to the H-2A locus using congenic and intra-H-2-recombinant strains. However, background (non-H-2) genetic factors substantially influenced both the response rate and the titer of ANoA/AFA attained. Strains bearing H-2As on the SJL and A backgrounds (SJL, A.SW, A.TH) showed 100% response rate and high ANoA titers (3750 +/- 246, mean reciprocal titer +/- SEM), whereas H-2As mice on the B10 background (B10.S) showed 60% response rate and significantly lower ANoA titers (1170 +/- 305) in the responding mice. Expression of H-2E [B10.S(9R) mice] further reduced the response rate (22%) and the ANoA titer (640 +/- 0). A suppressive effect on the B10 background has previously been observed in mercury treatment, but the effect was stronger in silver-treated mice. Two major differences were noted between silver- and mercury-induced murine autoimmunity. First, silver-treated mice did not show elevated titers of other autoantibody specificities, specifically not of antichromatin and anti-histone antibodies, which develop in mercury-treated SJL mice.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Animals
  • Antibodies, Antinuclear
  • Autoimmunity
  • Blood Vessels
  • Cell Nucleolus
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Glomerular Mesangium
  • Histones
  • Immunoblotting
  • Mice
  • Mice, Inbred Strains
  • Silver Nitrate
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Identity

International Standard Serial Number (ISSN)

  • 0090-1229

Digital Object Identifier (DOI)

  • 10.1006/clin.1995.1155

PubMed ID

  • 7586739
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Additional Document Info

start page

  • 291

end page

  • 297

volume

  • 77

issue

  • 3

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