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The first crystal structure of a macromolecular assembly under high pressure: Cpmv at 330 mpa

Academic Article
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Overview

authors

  • Girard, E.
  • Kahn, R.
  • Mezouar, M.
  • Dhaussy, A. C.
  • Lin, T. W.
  • Johnson Jr., John
  • Fourme, R.

publication date

  • 2005

journal

  • Biophysical Journal  Journal

abstract

  • The structure of cubic Cowpea mosaic virus crystals, compressed at 330 MPa in a diamond anvil cell, was refined at 2.8 A from data collected using ultrashort-wavelength (0.331 A) synchrotron radiation. With respect to the structure at atmospheric pressure, order is increased with lower Debye Waller factors and a larger number of ordered water molecules. Hydrogen-bond lengths are on average shorter and the cavity volume is strongly reduced. A tentative mechanistic explanation is given for the coexistence of disordered and ordered cubic crystals in crystallization drops and for the disorder-order transition observed in disordered crystals submitted to high pressure. Based on such explanation, it can be concluded that pressure would in general improve, albeit to a variable extent, the order in macromolecular crystals.

subject areas

  • Biophysics
  • Capsid
  • Comovirus
  • Crystallography, X-Ray
  • Hydrogen Bonding
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Conformation
  • Pressure
  • Proteins
  • Software
  • Surface Properties
  • Synchrotrons
  • Temperature
  • Water
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Identity

PubMed Central ID

  • PMC1305503

International Standard Serial Number (ISSN)

  • 0006-3495

Digital Object Identifier (DOI)

  • 10.1529/biophysj.104.058636

PubMed ID

  • 15731378
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Additional Document Info

start page

  • 3562

end page

  • 3571

volume

  • 88

issue

  • 5

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