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Simultaneous binding of two proteins to opposite sides of a single transfer RNA

Academic Article
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Overview

authors

  • Nomanbhoy, T.
  • Morales, A. J.
  • Abraham, A. T.
  • Vortler, C. S.
  • Giege, R.
  • Schimmel, Paul

publication date

  • April 2001

journal

  • Nature Structural Biology  Journal

abstract

  • Transfer RNA (tRNA) is a small nucleic acid (typically 76 nucleotides) that forms binary complexes with proteins, such as aminoacyl tRNA synthetases (RS) and Trbp111. The latter is a widely distributed structure-specific tRNA-binding protein that is incorporated into cell signaling molecules. The structure of Trbp111 was modeled onto to the outer, convex side of the L-shaped tRNA. Here we present RNA footprints that are consistent with this model. This binding mode is in contrast to that of tRNA synthetases, which bind to the inside, or concave side, of tRNA. These opposite locations of binding for these two proteins suggest the possibility of a ternary complex. The formation of a tRNA synthetase--tRNA--Trbp111 ternary complex was detected by two independent methods. The results indicate that the tRNA is sandwiched between the two protein molecules. A thermodynamic and functional analysis is consistent with the tRNA retaining its native structure in the ternary complex. These results may have implications for how the translation apparatus is linked to other cellular machinery.

subject areas

  • Acylation
  • Bacteria
  • Bacterial Proteins
  • Binding Sites
  • Electrophoresis
  • Escherichia coli
  • Isoleucine-tRNA Ligase
  • Models, Molecular
  • Nuclease Protection Assays
  • Nucleic Acid Conformation
  • Protein Binding
  • Protein Conformation
  • RNA Probes
  • RNA, Transfer
  • RNA, Transfer, Asp
  • RNA, Transfer, Leu
  • RNA, Transfer, Met
  • RNA-Binding Proteins
  • Spectrometry, Fluorescence
  • Thermodynamics
  • Yeasts
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Identity

International Standard Serial Number (ISSN)

  • 1072-8368

Digital Object Identifier (DOI)

  • 10.1038/86228

PubMed ID

  • 11276256
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Additional Document Info

start page

  • 344

end page

  • 348

volume

  • 8

issue

  • 4

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