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Isolation and characterization of a homogeneous isomeric species of carcinoembryonic antigen - cea-s

Academic Article
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Overview

authors

  • Plow, E. F.
  • Edgington, Thomas

publication date

  • 1975

journal

  • International Journal of Cancer  Journal

abstract

  • A single homogeneous isomeric species of carcinoembryonic antigen was isolated by reference to solubility in 0.9 M perchloric acid, isoelectric focusing, molecular exclusion chromatography, ion exchange chromatography, passage through immuno-absorbants, and isopyknic density gradient ultracentrifugation. The final product, representing approximately 1.8% of the perchloric acid soluble glycoprotein of the tumor, is homogeneous and devoid of other proteins by polyacrylamide gel electrophoresis. This single species of carcinoembryonic antigen, CEA-S, has a sedimentation velocity of 6.6, a diffusion constant of 3.05 times 10-minus 7 cm-2/sec, a mean Stokes radius of 65 A, a density of 1.41 ml/g in cesium chloride and an estimated molecular weight of 181,000, and it is devoid of detectable A or B blood-group antigens. Immunochemical studies demonstrate qualitative similarities between CEA-S and conventional carcinoembryonic antigens; however, competitive inhibition analyses demonstrate significant quantitative immunochemical differences between CEA-S and preparations of carcinoembryonic antigen. These results are consistent with the concept that CEA-S is an immunochemical isomer of carcinoembryonic antigen.

subject areas

  • Adenocarcinoma
  • Binding, Competitive
  • Carcinoembryonic Antigen
  • Centrifugation, Density Gradient
  • Chemical Fractionation
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Colonic Neoplasms
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Isoelectric Focusing
  • Liver Neoplasms
  • Molecular Weight
  • Neoplasm Metastasis
  • Radioimmunoassay
  • Ultracentrifugation
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Identity

International Standard Serial Number (ISSN)

  • 0020-7136

Digital Object Identifier (DOI)

  • 10.1002/ijc.2910150506

PubMed ID

  • 1140871
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Additional Document Info

start page

  • 748

end page

  • 761

volume

  • 15

issue

  • 5

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