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Myocyte enhancer factor 2C as a neurogenic and antiapoptotic transcription factor in murine embryonic stem cells

Academic Article
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Overview

authors

  • Li, Z.
  • McKercher, S. R.
  • Cui, J.
  • Nie, Z. G.
  • Soussou, W.
  • Roberts, Amanda
  • Sallmen, T.
  • Lipton, J. H.
  • Talantova, M.
  • Okamoto, S. I.
  • Lipton, Stuart

publication date

  • June 2008

journal

  • Journal of Neuroscience  Journal

abstract

  • Cell-based therapies require a reliable source of cells that can be easily grown, undergo directed differentiation, and remain viable after transplantation. Here, we generated stably transformed murine ES (embryonic stem) cells that express a constitutively active form of myocyte enhancer factor 2C (MEF2CA). MEF2C has been implicated as a calcium-dependent transcription factor that enhances survival and affects synapse formation of neurons as well as differentiation of cardiomyocytes. We now report that expression of MEF2CA, both in vitro and in vivo, under regulation of the nestin enhancer effectively produces "neuronal" progenitor cells that differentiate into a virtually pure population of neurons. Histological, electrophysiological, and behavioral analyses demonstrate that MEF2C-directed neuronal progenitor cells transplanted into a mouse model of cerebral ischemia can successfully differentiate into functioning neurons and ameliorate stroke-induced behavioral deficits.

subject areas

  • Animals
  • Apoptosis
  • Brain Ischemia
  • Brain Tissue Transplantation
  • Cell Differentiation
  • Cell Line, Transformed
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Embryonic Stem Cells
  • Gene Expression Regulation
  • Intermediate Filament Proteins
  • MEF2 Transcription Factors
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myogenic Regulatory Factors
  • Nerve Degeneration
  • Nerve Tissue Proteins
  • Nestin
  • Neurons
  • Organ Culture Techniques
  • Stem Cell Transplantation
  • Stem Cells
  • Transcription Factors
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Research

keywords

  • MEF2C
  • antiapoptotic
  • embryonic stem cells
  • neurogenesis
  • neuronal progenitor cell
  • transplantation
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Identity

PubMed Central ID

  • PMC2679693

International Standard Serial Number (ISSN)

  • 0270-6474

Digital Object Identifier (DOI)

  • 10.1523/jneurosci.0134-08.2008

PubMed ID

  • 18579729
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Additional Document Info

start page

  • 6557

end page

  • 6568

volume

  • 28

issue

  • 26

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